Figure 3. Cilia are completely resorbed... | Rockefeller University Press

Figure 3.

$Cilia are completely resorbed during mitosis in GC progenitors. (A) The cell cycle status of each cell in the EGL of the P7 SEM volume was determined and annotated. The fraction of cells in each phase with primary cilia is quantified. (B) The length of each EGL GC cilium is plotted by cell cycle phase. G1 and G0 cells cannot be distinguished; both have two centrioles. (C) Serial scanning EM images of cilia from four individual cells with duplicating centrioles that indicate the cells are in S phase or G2. Cilium length is noted in the upper right corner and procentrioles visible in the same section are indicated with a red crescent. (D) Serial sections of a non-ciliated mother centriole and procentriole in a G2 cell in the EGL. (E) Representative SEM images of non-ciliated centrioles in GC progenitors in each stage of mitosis. (F) Serial sections of mother centrioles from late-stage cytokinesis cells with a ciliary vesicle (upper) or nascent cilium (lower). (G) A model of the process of cilia resorption using representations of the cilia presented in C–F. Prior to mitosis, cilia disassemble, are absent during mitosis, and reappear late in cytokinesis.$

Cilia are completely resorbed during mitosis in GC progenitors. (A) The cell cycle status of each cell in the EGL of the P7 SEM volume was determined and annotated. The fraction of cells in each phase with primary cilia is quantified. (B) The length of each EGL GC cilium is plotted by cell cycle phase. G1 and G0 cells cannot be distinguished; both have two centrioles. (C) Serial scanning EM images of cilia from four individual cells with duplicating centrioles that indicate the cells are in S phase or G2. Cilium length is noted in the upper right corner and procentrioles visible in the same section are indicated with a red crescent. (D) Serial sections of a non-ciliated mother centriole and procentriole in a G2 cell in the EGL. (E) Representative SEM images of non-ciliated centrioles in GC progenitors in each stage of mitosis. (F) Serial sections of mother centrioles from late-stage cytokinesis cells with a ciliary vesicle (upper) or nascent cilium (lower). (G) A model of the process of cilia resorption using representations of the cilia presented in C–F. Prior to mitosis, cilia disassemble, are absent during mitosis, and reappear late in cytokinesis.