![SBP-1, LPIN-1, SMS-1, and HMGR-1 promote AC invasive protrusion growth. (A) Maximum intensity z-projected time-lapse fluorescence images showing the formation of the AC invasive protrusion (mCherry::PLCδPH, cyan) in a control and sbp-1 RNAi knockdown animal (BM, orange dashed lines). Time points indicate h::min. Isosurfaces of the AC invasive protrusions are shown below (see Video 1). The 0:45 min timepoint for control and sbp-1 RNAi is also shown in Fig. 2 A. (B–E) Left: Line graphs of invasive protrusion volume over time in control (cyan) animals and in animals after (B) sbp-1, (C) lpin-1, (D) sms-1, and (E) hmgr-1 AC-specific RNAi treatment (magenta; see Videos 1, 2, 6, and 7). Right in B–E: Boxplots of the AC invasive protrusion growth rates. In this and subsequent figures, box edges indicate the 25th and 75th percentiles, whiskers the maximum and minimum values, and the line inside each box the median value. AC protrusion growth rate over 60 or 64 min was calculated ([B] n = 7 control and 8 sbp-1 RNAi animals; [C] n = 7 control and 5 lpin-1 RNAi animals; [D] n = 5 control and 5 sms-1 RNAi animals; [E] n = 9 control and 7 hmgr-1 RNAi animals ** P ≤ 0.01, P = 0.1490, Mann–Whitney U test). All data are from two or more replicates. Scale bar, 5 µm.](https://cdn.rupress.org/rup/content_public/journal/jcb/223/10/10.1083_jcb.202402035/2/jcb_202402035_figs2.png?Expires=1771484148&Signature=nDFyB4iBl3g01FPAa1BO74RnRcVj43kfJKLEMQfcuJML0YR-T8d-J6vgAKl4s0HfTPafLzQuZsw3kR664QtapwvdA20lVs8YmydejDU7BAWx8jGBwd0XIiYDvJlDLGX3gP4rNxDQTb1WUF5dPw22RqtvjgLkO7NsOqxagmRUJ-UE8CglphQbL3AEf7KhUpvE7Ztq82ixxaiCZe1hYwv~Fb02NjRDHA1UYN0ia~Qv1z9JbXRCzLboobVnDlk57eQ7e-AYMCEapq4I-xiE-MXC0oFA8LX~ePFGmBboDwUdgcycDqS-WKyoEhVHoq28ASvfuuZWPgOV9bG1hLmKGa4jQg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SBP-1, LPIN-1, SMS-1, and HMGR-1 promote AC invasive protrusion growth. (A) Maximum intensity z-projected time-lapse fluorescence images showing the formation of the AC invasive protrusion (mCherry::PLCδPH, cyan) in a control and sbp-1 RNAi knockdown animal (BM, orange dashed lines). Time points indicate h::min. Isosurfaces of the AC invasive protrusions are shown below (see Video 1). The 0:45 min timepoint for control and sbp-1 RNAi is also shown in Fig. 2 A. (B–E) Left: Line graphs of invasive protrusion volume over time in control (cyan) animals and in animals after (B) sbp-1, (C) lpin-1, (D) sms-1, and (E) hmgr-1 AC-specific RNAi treatment (magenta; see Videos 1, 2, 6, and 7). Right in B–E: Boxplots of the AC invasive protrusion growth rates. In this and subsequent figures, box edges indicate the 25th and 75th percentiles, whiskers the maximum and minimum values, and the line inside each box the median value. AC protrusion growth rate over 60 or 64 min was calculated ([B] n = 7 control and 8 sbp-1 RNAi animals; [C] n = 7 control and 5 lpin-1 RNAi animals; [D] n = 5 control and 5 sms-1 RNAi animals; [E] n = 9 control and 7 hmgr-1 RNAi animals ** P ≤ 0.01, P = 0.1490, Mann–Whitney U test). All data are from two or more replicates. Scale bar, 5 µm.
