Pak1-mEGFP and CRIB-GFP dynamics under different conditions. (A) Pak1-mEGFP dynamics in DMSO and CK-666 treated cells. Magenta arrows indicate peaks of fluorescence intensity at that cell end. (B) FRAP analysis of Pak1-mEGFP recovery after photobleaching half the cell end. Magenta boxes indicate the half of the cell end that has been photobleached. Magenta arrows show when half of the final fluorescence has recovered. Blue arrows indicate when the fluorescence recovery plateaus. (C) Dynamics of CRIB-GFP and Pak1-mEGFP were observed in myo1+ and myo1∆ cells. Magenta arrows indicate peaks of fluorescent intensity at that cell end. Oscillation montages clarified and denoised, made in NIS elements. Scale bars, 10 µm.