Endocytic mutant myo1∆ cells show disrupted Cdc42 activation dynamics similar to CK-666 treatment. (A) CRIB-3xGFP dynamics were observed in myo1+ and myo1∆ cells. (B) Correlation coefficients of active Cdc42 oscillations between cell ends in myo1+ cells and myo1∆ mutants (n ≥ 10 cells). (C) Scd1-mNG localization in myo1+ and myo1∆ cells. Asterisks indicate Scd1-mNG localization at the brighter cell end. (D) Quantification of Scd1-mNG accumulation at cell ends in myo1+ and myo1∆ cells (N = 3, n ≥ 10 cells). (E) Pak1-mEGFP localization in myo1+ and myo1∆ cells. Asterisks indicate Pak1-mEGFP localization at the brighter cell end. (F) Quantification of Pak1-mEGFP accumulation at cell ends in myo1+ cells and myo1∆ mutants (N = 3, n ≥ 10 cells). (G) Pak1-mEGFP dynamics at cell ends in myo1+ and myo1∆ cells. (H) Quantification of the extent of Pak1-mEGFP fluctuation in myo1+ and myo1∆ cells (N = 3, n ≥ 10 cells). Scale bar, 10 µm. P value, *<0.05, **<0.0085, Student’s t test.