Loss of all F-actin structures causes a stress response but CK-666 treatment alone does not. (A) CRIB-3xGFP localization in for3⁺ and for3∆ cells treated with DMSO and CK-666. (B) Quantification of the number of cells with depolarized CRIB-3xGFP localization. (C) Gef1-mNG localization in for3+ and for3∆ cells treated with DMSO and CK-666. (D) Quantification of the number of cells with depolarized Gef1-mNG localization. Scale bar, 10 µm. n.s., not significant; P value, ****<0.0001, one-way ANOVA, followed by Tukey’s multiple comparison test.