Branched actin is required for competition for active Cdc42 between growing ends. (A) Cdc42 dynamics in CRIB-3xGFP expressing cells treated with DMSO, Lat-A, CK666, and in for3Δ mutants. Red arrowheads mark the site of Cdc42 activation. (B) Representative quantification of CRIB-3xGFP oscillations in DMSO and Lat-A-treated cells. (C) Representative quantification of CRIB-3xGFP oscillations in for3∆ and CK-666-treated cells. (D) Correlation coefficient of active Cdc42 oscillations between cell ends (n ≥ 9 cells). Scale bar, 10 μm. n.s., not significant; P value, *<0.05, **<0.001, ****<0.0001, one-way ANOVA, followed by Tukey’s multiple comparison test.