Figure S1.
REEP5 specifically interacts with MFN1/2, and the REEP5 knockdown affects mitochondrial morphology. (A and B) Co-IP analysis of Myc-tagged Rtn1-4 or REEP5 with Flag-MFN1/2 from HEK293T cells. (C) Confocal images of mitochondrial distribution in U2OS cells transfected with siRtn3 or siREEP5. siRNA efficiencies were analyzed by immunoblotting. Endogenous mitochondria were immunostained with anti-Tom20 and the nucleus was labeled with DAPI. Bar: 10 µm (whole cell view); 5 µm (zoomed-in images). (D) Mitochondrial morphology from U2OS cells expressing different amounts of HaloTag-MFN2 as indicated. MFN2 protein levels were detected by immunoblotting. Cell number for each transfection, 4 × 105. Bar: 10 µm. Source data are available for this figure: SourceData FS1.

REEP5 specifically interacts with MFN1/2, and the REEP5 knockdown affects mitochondrial morphology. (A and B) Co-IP analysis of Myc-tagged Rtn1-4 or REEP5 with Flag-MFN1/2 from HEK293T cells. (C) Confocal images of mitochondrial distribution in U2OS cells transfected with siRtn3 or siREEP5. siRNA efficiencies were analyzed by immunoblotting. Endogenous mitochondria were immunostained with anti-Tom20 and the nucleus was labeled with DAPI. Bar: 10 µm (whole cell view); 5 µm (zoomed-in images). (D) Mitochondrial morphology from U2OS cells expressing different amounts of HaloTag-MFN2 as indicated. MFN2 protein levels were detected by immunoblotting. Cell number for each transfection, 4 × 105. Bar: 10 µm. Source data are available for this figure: SourceData FS1.

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