Figure 5. T cells follow the paths of... | Rockefeller University Press

Figure 5.

$T cells follow the paths of each other. (A) Comparison of the mobile versus immobile fraction of Th1 cells in confinement between an agarose gel and a polyacrylamide gel coated with FN, ICAM-1, or uncoated. (B) Comparison of actual displacement of the cells from A. Mobile cells are defined by a minimum displacement of 10 µm. (C) Roseplots showing Th1 cell tracks of cells in A and B. The colormap represents the cell's average velocity. (D) Individual cell tracking shows cells extending their path and accelerating after each pass. (E) Individual cell tracking shows three cells extending their paths and a fourth cell (orange) reusing those previously made paths and accelerating while doing so. Colors represent the cell's average velocity. (F) Measurement of the frequency of cells following each other. (G–J) Measurement of the change in velocity of cells as they encounter previous paths of migrating T cells confined between an agarose gel and a polyacrylamide gel coated with (G) FN, (H) ICAM-1, (I) DMSO, or (J) 10 µM Pan MMP Inhibitor. Number of biological replicates (colored dots): (A and B) Uncoated, n = 3; FN, n = 4; ICAM-1, n = 3. (F–H) FN n = 4; ICAM-1 n = 3. (I–J) FN + DMSO n = 2; FN + MMP inhibitor, n = 2. Each replicates represent the average of 5–10 fields of view (gray dots = average of each fields of view). Statistical tests: (A and B) Two-tail unpaired parametric t test; (G–J) One-tail paired parametric t test.$

T cells follow the paths of each other. (A) Comparison of the mobile versus immobile fraction of Th1 cells in confinement between an agarose gel and a polyacrylamide gel coated with FN, ICAM-1, or uncoated. (B) Comparison of actual displacement of the cells from A. Mobile cells are defined by a minimum displacement of 10 µm. (C) Roseplots showing Th1 cell tracks of cells in A and B. The colormap represents the cell's average velocity. (D) Individual cell tracking shows cells extending their path and accelerating after each pass. (E) Individual cell tracking shows three cells extending their paths and a fourth cell (orange) reusing those previously made paths and accelerating while doing so. Colors represent the cell's average velocity. (F) Measurement of the frequency of cells following each other. (G–J) Measurement of the change in velocity of cells as they encounter previous paths of migrating T cells confined between an agarose gel and a polyacrylamide gel coated with (G) FN, (H) ICAM-1, (I) DMSO, or (J) 10 µM Pan MMP Inhibitor. Number of biological replicates (colored dots): (A and B) Uncoated, n = 3; FN, n = 4; ICAM-1, n = 3. (F–H) FN n = 4; ICAM-1 n = 3. (I–J) FN + DMSO n = 2; FN + MMP inhibitor, n = 2. Each replicates represent the average of 5–10 fields of view (gray dots = average of each fields of view). Statistical tests: (A and B) Two-tail unpaired parametric t test; (G–J) One-tail paired parametric t test.