Th1 cells form FAs containing vinculin and paxillin. (A–D) Representative Th1 cells expressing Vinculin-eGFP in confinement on (A) ICAM-1, (B) Fibronectin, (C) PLL, and (D) PLL-PEG. (E) A Th1 expressing Vinculin-eGFP in confinement on a micropatterned substrate. The left portion of the field of view is fibronectin-coated and the right portion is passivated. Arrows indicate vinculin accumulation in FAs. (F) Representative fixed images of Th1 cells in confinement, stained with an anti-paxillin antibody to mark endogenous adhesions, phalloidin to visualize actin, and DAPI to mark the nucleus.