Figure 1. Th1 migration, velocity, and... | Rockefeller University Press

Figure 1.

$Th1 migration, velocity, and displacement are regulated by the environment composition and geometry. (A) Th1 cell tracking on ICAM-1 and FN in unconfined versus confined (5 µm) environments. The colormap represents the cell's average velocity in µm/sec. (B) Comparison of the actual displacement, defined as the distance between the starting and end points of a track, of the mobile cells in A. (C) Graphical representation of the different substrates used and their properties. (D) The mobile versus immobile fraction of cells in confinement on ICAM-1, FN, PLL, and Passivated surfaces. Mobile cells are defined by a minimum displacement of 10 µm. (E and F) The effective velocities (E) and actual displacement (F) of mobile cells in D. (H) Cell tracking on a FN micropatterned substrate. Gray = FN, White = Passivated. The colors represent the instantaneous velocities of each cell at a given time point. (I) Kymographs of the regions identified in H show cells stalling on the FN patterned stripes. (J) Snapshots of the movie from H. A single cell is highlighted in orange to illustrate its trajectory over time. Number of biological replicates (colored dots): (B) FN, n = 3; ICAM-1, n = 4. (D-F) FN, n = 8; ICAM-1, n = 6; PLL, n = 6; Passivated, n = 7. Each replicate represents the average of 5–10 fields of view (gray dots = average of each field of view), which each contain ∼250 cells. Statistical tests: (B) one-tail paired parametric t test; (D–F) two-tailed unpaired parametric t test.$

Th1 migration, velocity, and displacement are regulated by the environment composition and geometry. (A) Th1 cell tracking on ICAM-1 and FN in unconfined versus confined (5 µm) environments. The colormap represents the cell's average velocity in µm/sec. (B) Comparison of the actual displacement, defined as the distance between the starting and end points of a track, of the mobile cells in A. (C) Graphical representation of the different substrates used and their properties. (D) The mobile versus immobile fraction of cells in confinement on ICAM-1, FN, PLL, and Passivated surfaces. Mobile cells are defined by a minimum displacement of 10 µm. (E and F) The effective velocities (E) and actual displacement (F) of mobile cells in D. (H) Cell tracking on a FN micropatterned substrate. Gray = FN, White = Passivated. The colors represent the instantaneous velocities of each cell at a given time point. (I) Kymographs of the regions identified in H show cells stalling on the FN patterned stripes. (J) Snapshots of the movie from H. A single cell is highlighted in orange to illustrate its trajectory over time. Number of biological replicates (colored dots): (B) FN, n = 3; ICAM-1, n = 4. (D-F) FN, n = 8; ICAM-1, n = 6; PLL, n = 6; Passivated, n = 7. Each replicate represents the average of 5–10 fields of view (gray dots = average of each field of view), which each contain ∼250 cells. Statistical tests: (B) one-tail paired parametric t test; (D–F) two-tailed unpaired parametric t test.