MAP1B and Tks5 are mutually dependent for their binding to microtubules. (A) Colocalization between GFP-Tks5 and α-tubulin in MAP1B siRNA-transfected MDA/GFP-Tks5 cells. Left: Representative immunofluorescence images. Arrowheads indicate residual GFP-Tks5 on microtubules in MAP1B-depleted cells. Right: Quantification of colocalization between GFP-Tks5 and α-tubulin. n = 36 cells from three independent experiments. (B) The proximity between Tks5 and α-tubulin in MAP1B siRNA-transfected MDA-MB-231 cells. Right: Quantification of total PLA dots’ area per cell. n = 30 cells from three independent experiments. (C) Immunoblotting of Tks5 and MAP1B-LC1 in Tks5 siRNA-transfected MDA-MB-231 cells. (D) The proximity between MAP1B-LC1 and α-tubulin in Tks5 siRNA-transfected MDA-MB-231 cells. Right: Quantification of total PLA dots’ area per cell. n = 30 cells from three independent experiments. (A–C) Data are presented as boxes containing the first and third quartiles. The whiskers indicate the maxima and minima after outlier removal. P values were determined using an unpaired two-tailed Student’s t test. ***, P < 0.001; n.s., not significant. Scale bars, 10 μm. Source data are available for this figure: SourceData F6.