PC4-KO inhibits liver cancer cell growth in vitro and in vivo. Relative to Fig. 5. (A) Western blot showing PC4 protein expression levels in Huh7 cells with control or PC4-KO. Data are generated from n = 3 biological replicates. (B) Cell viability analysis by CCK8 assay in Huh7 cells with control or PC4-KO. Data were generated from n = 5 biological replicates. (C) Colony assay in Huh7 cells with control or PC4-KO. Data are generated from n = 3 biological replicates. (D) Soft agar assay in Huh7 cells with control or PC4-KO. Scale bar = 300 μm. Data are generated from n = 4 biological replicates. (E) EdU assay in Huh7 cells with control or PC4 knockout. scale bar = 75 μm. Data are generated from n = 3 biological replicates. (F) Photographs of dissected tumors and tumor weight in the nude mice subcutaneously injected with Huh7 cell with control or PC4-KO. Data are generated from n = 5 biological replicates. (G) Tumor volume in the nude mice subcutaneously injected with Huh7 cell with control or PC4-KO. Data were generated from n = 5 biological replicates. All quantifications were shown as means ± SD (unpaired Student’s t test); error bars represent SD. **P < 0.01, ***P < 0.001, ****P < 0.0001. A is representative of three independent experiments. Source data are available for this figure: SourceData FS4.