Figure 1.
MAP1B is highly expressed in TNBC. (A) Immunoblotting of MAP1B, Tks5, and MT1-MMP in TNBC and non-TNBC cell lines. Upper panel: Representative results. Lower panel: Quantification of the blots. The values indicate the mean ± SEM normalized by α-tubulin levels (MAP1B-LC1: n = 9 experiments in all cell lines, except for n = 6 experiments in SH-SY5Y; Tks5: n = 8 experiments in all cell lines, except for n = 5 experiments in SH-SY5Y; MT1-MMP: n = 9 experiments in all cell lines, except for n = 6 experiments in SH-SY5Y). TN, TNBC; LA, luminal A; and HE, HER2-positive cells. (B) RT-qPCR analysis of MAP1B, Tks5, and MT1-MMP mRNA levels in TNBC and non-TNBC cell lines. The expression data are presented without being normalized by any of the housekeeping genes. The normalized data are indicated in Fig. S1 C. The values indicate the mean ± SEM (n = 6 experiments). (C) Immunohistochemistry of MAP1B-LC1 in primary tumors from patients with TNBC or luminal type BC. Upper panel: Representative images (Triple-negative, IN04, and Luminal, IN07; the detail inspections are shown in Fig. S2, B and C). Scale bar, 100 μm (luminal) and 10 μm (3+). Lower panel: Quantification of cell numbers categorized into four classes based on the staining level (negative, 1+, 2+, and 3+). Data are presented as boxes containing the first and third quartiles. The whiskers indicate the maxima and minima after outlier removal. The numbers over each whisker in the graph indicate means and medians, respectively. TNBC, n = 6 patients; non-TNBC, n = 5 patients. (D) Kaplan–Meier plots of patients with breast cancer with MAP1B, Tks5, and MT1-MMP levels. Upper panel: All subtypes. Lower panel: TNBC. Datasets were obtained from the KM plotter breast cancer database. HR indicates a hazard ratio. (A and B) P values were determined using one-way ANOVA with Tukey’s multiple comparisons. (C) P value was determined using a Wilcoxon’s signed rank sum test. (D) P values were determined using a log-rank test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; n.s., not significant. Source data are available for this figure: SourceData F1.

MAP1B is highly expressed in TNBC. (A) Immunoblotting of MAP1B, Tks5, and MT1-MMP in TNBC and non-TNBC cell lines. Upper panel: Representative results. Lower panel: Quantification of the blots. The values indicate the mean ± SEM normalized by α-tubulin levels (MAP1B-LC1: n = 9 experiments in all cell lines, except for n = 6 experiments in SH-SY5Y; Tks5: n = 8 experiments in all cell lines, except for n = 5 experiments in SH-SY5Y; MT1-MMP: n = 9 experiments in all cell lines, except for n = 6 experiments in SH-SY5Y). TN, TNBC; LA, luminal A; and HE, HER2-positive cells. (B) RT-qPCR analysis of MAP1B, Tks5, and MT1-MMP mRNA levels in TNBC and non-TNBC cell lines. The expression data are presented without being normalized by any of the housekeeping genes. The normalized data are indicated in Fig. S1 C. The values indicate the mean ± SEM (n = 6 experiments). (C) Immunohistochemistry of MAP1B-LC1 in primary tumors from patients with TNBC or luminal type BC. Upper panel: Representative images (Triple-negative, IN04, and Luminal, IN07; the detail inspections are shown in Fig. S2, B and C). Scale bar, 100 μm (luminal) and 10 μm (3+). Lower panel: Quantification of cell numbers categorized into four classes based on the staining level (negative, 1+, 2+, and 3+). Data are presented as boxes containing the first and third quartiles. The whiskers indicate the maxima and minima after outlier removal. The numbers over each whisker in the graph indicate means and medians, respectively. TNBC, n = 6 patients; non-TNBC, n = 5 patients. (D) Kaplan–Meier plots of patients with breast cancer with MAP1B, Tks5, and MT1-MMP levels. Upper panel: All subtypes. Lower panel: TNBC. Datasets were obtained from the KM plotter breast cancer database. HR indicates a hazard ratio. (A and B) P values were determined using one-way ANOVA with Tukey’s multiple comparisons. (C) P value was determined using a Wilcoxon’s signed rank sum test. (D) P values were determined using a log-rank test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; n.s., not significant. Source data are available for this figure: SourceData F1.

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