RNA-seq data analysis and RT-qPCR in TNBC cell lines and patients. (A) Volcano plots showing differential expressions of microtubule/actin-binding proteins between TNCB and other breast cancer subtypes in cell lines (data from GEO accession no. GSE75168) and patients (data from GEO accession no. GSE75688). (B) RT-qPCR analysis of housekeeping genes, HPRT1, GAPDH, and RPLP2, mRNA levels. (C) MAP1B, Tks5, and MT1-MMP mRNA levels normalized to the housekeeping genes’ levels. (B and C) The values indicate the mean ± SEM (n = 6 experiments). P values were determined using one-way ANOVA with Tukey’s multiple comparisons. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; n.s., not significant.