Oligomerized Spc105 exhibits dynein- and RZZ-dependent poleward movement. (A) Disorder plot of Spc105 and schematics of full-length Spc105 and truncations fused to mCherry-Cry2. (B) Schematic of the photo-oligomerization assay with a representative example of its application in living cells. (C) Schematic of the activation/imaging protocol applied to visualize oligomers in mitotic cells. (D) Representative example of photo-oligomerized 1–400-mCherry-Cry2 streaming poleward on a mitotic spindle with accompanying kymograph. (E) Representative control and DHC-depleted cells expressing 1–1722-mCherry-Cry2 and subjected to the activation/imaging protocol. (F) Quantification of the distribution of 1–1722 oligomers across mitotic spindles in control (red) and DHC-depleted (cyan) cells 3-min post activation (control, n = 10 spindles; DHC RNAi, n = 12 spindles). (G) Representative control and Rod-depleted cells expressing 1–1722-mCherry-Cry2 and subjected to the activation/imaging protocol. (H) Quantification of the distribution of 1–1722 oligomers across mitotic spindles in control (red) and Rod-depleted (cyan) cells 3-min post activation (control, n = 9 spindles; Rod RNAi, n = 9 spindles). Error bars are SEM. Scale bars, 10 μm; kymograph; 5 μm (horizontal) and 1 min (vertical). Displayed times are min:s.