Figure S6.
Net movement of dextran and EGF endosomes over time. (a) Airyscan confocal images of dextran-AF647 (magenta, left), Rab7-GFP (green, center), and their merge in HeLa cells fixed 2 min (top), 15 min (middle), and 30 min (bottom) after introduction of dextran to the cells. (b) Airyscan confocal images of EGF-AF647 (magenta, left), Rab7-GFP (green, center), and their merge in HeLa cells fixed 2 min (top), 15 min (middle), and 30 min (bottom) after introduction of EGF to the cells. (c) Airyscan confocal images of dextran-AF647 (magenta, left), LAMP1-GFP (green, center), and their merge in HeLa cells fixed 2 min (top), 15 min (middle), and 30 min (bottom) after introduction of dextran to the cells. (d) Airyscan confocal images of EGF-AF647 (magenta, left), LAMP1-GFP (green, center), and their merge in HeLa cells fixed 2 min (top), 15 min (middle), and 30 min (bottom) after introduction of EGF to the cells. (e) Quantification (mean ± SD) of the distance of dextran and EGF vesicles from the membrane in cells fixed 15 min after the introduction of AF647-labeled dextran or EGF. Each dot represents the mean distance of several endosomes from an individual cell (n = 29 for dextran data, and n = 29 cells for EGF data, from n = 3 independent experiments). (f) Quantification (mean ± SD) of the distance of dextran and EGF vesicles from the membrane in cells fixed 30 min after the introduction of AF647-labeled dextran or EGF. Each dot represents the mean distance of several endosomes from an individual cell (n = 26 cells for dextran data, and n = 37 cells for EGF data, from n = 3 independent experiments). (g) Plot of the number of Rab7+ dextran vesicles compared to the number of dextran vesicles (left), and plot of number of Rab7+ EGF vesicles compared with the number of EGF vesicles (right) at 2, 15, and 30 min after introduction of AF647-labeled dextran or EGF to HeLa cells. (h) Plot of the number of LAMP1+ dextran vesicles compared with the number of dextran vesicles (left), and plot of the number of LAMP1+ EGF vesicles compared with the number of EGF vesicles (right) at 2, 15, and 30 min after introduction of AF647-labeled dextran or EGF to HeLa cells. Scale bars in a–d: 10 µm; error bars in e–h represent SD.

Net movement of dextran and EGF endosomes over time. (a) Airyscan confocal images of dextran-AF647 (magenta, left), Rab7-GFP (green, center), and their merge in HeLa cells fixed 2 min (top), 15 min (middle), and 30 min (bottom) after introduction of dextran to the cells. (b) Airyscan confocal images of EGF-AF647 (magenta, left), Rab7-GFP (green, center), and their merge in HeLa cells fixed 2 min (top), 15 min (middle), and 30 min (bottom) after introduction of EGF to the cells. (c) Airyscan confocal images of dextran-AF647 (magenta, left), LAMP1-GFP (green, center), and their merge in HeLa cells fixed 2 min (top), 15 min (middle), and 30 min (bottom) after introduction of dextran to the cells. (d) Airyscan confocal images of EGF-AF647 (magenta, left), LAMP1-GFP (green, center), and their merge in HeLa cells fixed 2 min (top), 15 min (middle), and 30 min (bottom) after introduction of EGF to the cells. (e) Quantification (mean ± SD) of the distance of dextran and EGF vesicles from the membrane in cells fixed 15 min after the introduction of AF647-labeled dextran or EGF. Each dot represents the mean distance of several endosomes from an individual cell (n = 29 for dextran data, and n = 29 cells for EGF data, from n = 3 independent experiments). (f) Quantification (mean ± SD) of the distance of dextran and EGF vesicles from the membrane in cells fixed 30 min after the introduction of AF647-labeled dextran or EGF. Each dot represents the mean distance of several endosomes from an individual cell (n = 26 cells for dextran data, and n = 37 cells for EGF data, from n = 3 independent experiments). (g) Plot of the number of Rab7+ dextran vesicles compared to the number of dextran vesicles (left), and plot of number of Rab7+ EGF vesicles compared with the number of EGF vesicles (right) at 2, 15, and 30 min after introduction of AF647-labeled dextran or EGF to HeLa cells. (h) Plot of the number of LAMP1+ dextran vesicles compared with the number of dextran vesicles (left), and plot of the number of LAMP1+ EGF vesicles compared with the number of EGF vesicles (right) at 2, 15, and 30 min after introduction of AF647-labeled dextran or EGF to HeLa cells. Scale bars in a–d: 10 µm; error bars in e–h represent SD.

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