Dextran and EGF vesicles display different pause times. (a) HILO image from a time-lapse of dextran vesicles in HeLa cells (left) and the corresponding kymograph (right). (b) HILO image from a time-lapse of EGF vesicles in HeLa cells (left) and the corresponding kymograph (right). (c) Plot of position versus time of dextran vesicles (gray) and EGF (green) in HeLa cells, and the predicted movement of endosomes after nocodazole treatment in the absence of MTs (magenta). The dashed black lines indicate absolute movement of 1 µm, with 67% of dextran vesicles and 57% of EGF vesicles moving <1 µm during the time-lapse imaging. (d) Probability distribution P−(τ) of the minus end–directed run times for dextran (gray) and EGF (green) vesicles. n = 196 tracks from two independent experiments. (e) Probability distribution P0(τ) of the pause times for dextran (gray) and EGF (green) vesicles. In a and b, “N” marks the location/direction of the nucleus. In d and e, the average run/pause time $(〈τ〉)$ for dextran and EGF vesicles are indicated. n = 214 tracks from two independent experiments.