The dynactin complex binds along the entire length of the MT. (a) Immunofluorescence image of MT (magenta) and p150 (green) obtained using spinning disk microscopy + SRRF. (b) Enlarged view of the area marked with the white rectangle in a and the line profile of p150 intensity along the length of a representative MT trace (white line is representative MT trace used for the line profile) from the plus end (“P”) to ∼6 µm from the plus end of the MT (“0”). (c) Immunofluorescence images of p62 (green) and p150 (magenta) obtained using spinning disk microscopy + SRRF. Enlarged views of the area marked with the white rectangle are shown as individual channel images and their merge to the right of the image. The white arrowheads indicate representative p150 spots that also contain p62. (d) Immunofluorescence image of MT (magenta) and p62 (green) obtained using spinning disk microscopy + SRRF. The white arrowheads indicate representative p62 spots that occur on the MT. (e) Plot of the probability of co-occurrence of p62 with p150, indicating a high likelihood of presence of the entire complex at a p150 spot. n = 59 cells across two independent experiments. (f) Plot of the probability of co-occurrence of p62 on the MT, which points to a high likelihood for the presence of the entire dynactin complex on the MT. n = 25 cells across one independent experiment. In a, c, and d, “N” marks the location/direction of the nucleus. Error bars in e and f represent SD.