Figure 7.
MCS-DETECT captures MERC changes induced by RRBP1 knockdown. (A) Volume-rendered MCS-DETECT views of HT-1080 WT and Gp78 KO cells treated with either siControl or siRRBP1. Mitochondria are labeled with TOMM20 (red) and MERCS are visualized in white. Bar = 10 µm whole cell; 1 µm insets. (B) Mitochondria surface coverage ratio and the number of contacts per sampled mitochondria window are shown for contact zones in HT-1080 WT and Gp78 KO cells treated with either siControl or siRRBP1. Averaged over cell, two-sided non-parametric Mann–Whitney test, n = 3 independent biological replicates, ≥30 cells/condition per experiment; *P < 0.05; **P < 0.01; ***P < 0.001. (C) HT-1080 cells transfected with either siControl or siRRBP1 were labeled with MitoView633. Integrated density of MitoView633 per cell was quantified. n = 3 independent biological replicates; >50 cells/condition per experiment; ***P < 0.0001; ****P < 0.0001; Tukey post hoc test.

MCS-DETECT captures MERC changes induced by RRBP1 knockdown. (A) Volume-rendered MCS-DETECT views of HT-1080 WT and Gp78 KO cells treated with either siControl or siRRBP1. Mitochondria are labeled with TOMM20 (red) and MERCS are visualized in white. Bar = 10 µm whole cell; 1 µm insets. (B) Mitochondria surface coverage ratio and the number of contacts per sampled mitochondria window are shown for contact zones in HT-1080 WT and Gp78 KO cells treated with either siControl or siRRBP1. Averaged over cell, two-sided non-parametric Mann–Whitney test, n = 3 independent biological replicates, ≥30 cells/condition per experiment; *P < 0.05; **P < 0.01; ***P < 0.001. (C) HT-1080 cells transfected with either siControl or siRRBP1 were labeled with MitoView633. Integrated density of MitoView633 per cell was quantified. n = 3 independent biological replicates; >50 cells/condition per experiment; ***P < 0.0001; ****P < 0.0001; Tukey post hoc test.

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