Figure 5.
Gp78 induces riboMERCs in HeLa cells. (A) Volume-rendered MCS-DETECT views of cells expressing ERmoxGFP and labeled for TOM20 (magenta) with contact sites overlaid (white) are shown for untransfected HeLa cells and HeLa cells overexpressing WT Gp78, Gp78 RM, and the OMM–ER linker. Bar = 10 µm whole cell; 1 µm insets). (B) Mitochondria surface coverage ratio and the number of contacts per sampled mitochondria window are shown for contact zones in the cells indicated above. Averaged over cell, two-sided non-parametric Mann–Whitney test, n = 3 independent biological replicates, ≥30 cells/condition per experiment; *P < 0.05; **P < 0.01; ***P < 0.001. (C) HeLa cells were transfected with EGFP (as a control), Gp78 WT, Gp78 RM, or the OMM–ER linker and labeled with MitoView 633. Integrated density of MitoView 633 per cell was quantified. n = 3 independent biological replicates; >35 cells/condition per experiment; ****P < 0.0001; Tukey post hoc test.

Gp78 induces riboMERCs in HeLa cells. (A) Volume-rendered MCS-DETECT views of cells expressing ERmoxGFP and labeled for TOM20 (magenta) with contact sites overlaid (white) are shown for untransfected HeLa cells and HeLa cells overexpressing WT Gp78, Gp78 RM, and the OMM–ER linker. Bar = 10 µm whole cell; 1 µm insets). (B) Mitochondria surface coverage ratio and the number of contacts per sampled mitochondria window are shown for contact zones in the cells indicated above. Averaged over cell, two-sided non-parametric Mann–Whitney test, n = 3 independent biological replicates, ≥30 cells/condition per experiment; *P < 0.05; **P < 0.01; ***P < 0.001. (C) HeLa cells were transfected with EGFP (as a control), Gp78 WT, Gp78 RM, or the OMM–ER linker and labeled with MitoView 633. Integrated density of MitoView 633 per cell was quantified. n = 3 independent biological replicates; >35 cells/condition per experiment; ****P < 0.0001; Tukey post hoc test.

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