Figure S2.
Identifying and filtering mitochondria. (A) For each contact, size and mean intensity of the adjacent mitochondria is plotted for two replicates, to indicate consistency across replicates. Note that this is by default larger than a segmentation method would compute. The size and mean intensity of mitochondria in HT-1080 and COS-7 cells present a clearly separable group of small, low-intensity mitochondria structures. To report results on what are clearly and unambiguously mitochondria, corresponding to the mitochondria observed by EM, mitochondrial structures smaller than thresholds 9 (ln size) and 0.2 (mean intensity) were eliminated. (B) 3D STED images show labeling of ER (green), mitochondria (magenta), and contact sites (white) of representative COS-7 and HT-1080 cells before and after mitochondrial filtering. Bar = 10 μM (insets: 1 μM).

Identifying and filtering mitochondria. (A) For each contact, size and mean intensity of the adjacent mitochondria is plotted for two replicates, to indicate consistency across replicates. Note that this is by default larger than a segmentation method would compute. The size and mean intensity of mitochondria in HT-1080 and COS-7 cells present a clearly separable group of small, low-intensity mitochondria structures. To report results on what are clearly and unambiguously mitochondria, corresponding to the mitochondria observed by EM, mitochondrial structures smaller than thresholds 9 (ln size) and 0.2 (mean intensity) were eliminated. (B) 3D STED images show labeling of ER (green), mitochondria (magenta), and contact sites (white) of representative COS-7 and HT-1080 cells before and after mitochondrial filtering. Bar = 10 μM (insets: 1 μM).

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