Effects of bridging activity mitotic chromosome structure at CFSs. (A) Analysis of bridging condensin effects on irregular FISH phenotypes, simulated by creating a longer loop within a regular bottlebrushed mitotic chromosome. 10 independent simulations with and without bridging condensins were performed, and the distance between two probes (violet segments in panel 1) was monitored. In the absence of bridges, the average 3D distance becomes slightly smaller (panel 2). (B) Study of the effects due to bridging mechanisms in the presence of cytological lesions. While the presence of bridges allows to clearly observe cytological lesions as in experiments (panel 1), their lack leads to a structure where the breakage is no longer visible (panel 2).