Adherent junctions are not altered after 24 h of vinculin suppression. (A) Scheme of the DNA vector used and the procedures followed in this figure. (B and C) Open-book captions of chicken NTs transfected at stage HH12 for 24 h with ShControl or ShVCL and PACT·RFP (labels centrioles, yellow) and stained with N-cadherin or phalloidin (cyan or gray). Cytoplasmic GFP (magenta) indicates transfection. Scale bar = 5 μm.