Vinculin controls velocity and directionality during basal to apical nuclear migration in the NT. (A–C) Formulas used and explicative schematic representations of the kinetic parameters studied from apicobasal and basoapical movements in NTs treated with blebbistatin or transfected with shVCL: mean velocity (mV, A), directional velocity (dV, B), and directionality ratio (dR, C). (A′–C′) Dot plots showing the apicobasal mean velocity (A′), directional velocity (B′), and directionality ratio (C′) of NTs treated with shControl + DMSO (control), 12.5 μM blebbistatin + shControl (blebbistatin) or DMSO + shVCL (shVCL). (A″–C″) Dot plots showing the baso-apical mean velocity (A″), directional velocity (B″), and directionality ratio (C″) of NTs treated with shControl + DMSO (control), 12.5 μM blebbistatin + shControl (blebbistatin), or DMSO + shVCL (shVCL). tD = total distance; sD = strait distance; T = time in minutes. ∞ = infinite. The black bars indicate the mean. One-way ANOVA plus Dunn’s multi-comparisons test, n = 18 cells per condition collected from two independent experiments using at least three embryos per condition. ns: non-significant, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001.