Basal to apical nuclear migration (BA ^INM ) in the NT is inhibited by vinculin downregulation and blebbistatin treatment. (A–C) Representative time-series of BA^INM of chicken NTs treated with DMSO and transfected with shControl (A), treated with 12.5 μM of blebbistatin (a myosin II inhibitor; dissolved in DMSO) and transfected with shControl (B), or treated with DMSO and transfected with shVCL (C). All NTs were also transfected with vectors expressing H2B·RFP (magenta) and membrane-GFP (green) to expose the nucleus and the contour of transfected cells. The blue dot indicates the tracked nucleus in each image. The entire trajectory followed by the tracked nucleus (cyan line) and its starting (white dot) and final (yellow dot) positions, are shown in the bottom panel of each treatment. Scale bar = 50 μm. (D–F) Drawings simulating the BA^INM in each experimental condition: DMSO + shControl (D, control), 12.5 μM blebbistatin + shControl or DMSO + shVCL (E, blebbistatin or vinculin downregulation). (G–I) Plots showing the individual nuclear basoapical trajectories of 18 cells for each experimental condition, each plot collects cells from two independent experiments: DMSO + shControl (F, control), 12.5 μM blebbistatin + shControl (G, blebbistatin), or DMSO + shVCL (H, shVCL). The X and Y axis represent the elapsed time and the distance to the apical pole, respectively. The apical pole position in the plot is represented by a dotted line.