Vinculin requires the interaction with actin to stabilize the apical acting complexes. (A) Scheme of the DNA vector and the procedures followed in this figure. (B–D) Images of transverse sections of chicken NTs transfected at stage HH12 for 24 h with empty vector, mVCL, or mVCL-ΔEx20 and stained with phalloidin (magenta or gray), nuclear GFP indicates transfection. C and D show two different slices for each construct. Scale bar = 50 μm. (E) Higher magnification caption of a slice transfected with mVCL. Actin rings protruding into the ventricle are indicated with arrows.