Efficiency of the sh-RNAs used to knock-down the studied ABPs. (A–F) The Sh-RNAs targeting each of the six ABPs studied were cloned into pSHIN vector. Two different Sh-RNAs were designed for each gene. The knockdown efficiency was tested by RT-qPCR in chicken embryonary fibroblasts (CEFs). Mean ± SEM. One-way ANOVA plus Dunnett’s multiple comparisons test, n = 3 (3 RT-qPCR reactions using three independent CEFs transfections) * = P < 0.005, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001.