Speculative model of Kinesin-1–regulated formation of cortical domains in syncytial embryos. Initially, Kinesin-1 functions in the organization of centrosomal (black line) and non-centrosomal microtubules (red lines) together with end-binding proteins EB1 and Patronin/Shot in a region closest to the centrosome, the cap region (blue). Either directly in a positive manner or indirectly via microtubules, the RhoGEF Pebble becomes restricted to the intercap region (green), where Rho signaling is activated, including Rho targets. In addition, Par-1 becomes enriched in intercap region. For maintenance, Par-1 and Patronin/Shot set up a mutual antagonism, in which Kinesin-1 and Rho signaling may or may not be involved.