Figure 8.
Mutual antagonism between Par-1 and Patronin/Shot. (A) Fixed embryos of indicated genotypes and stage stained for Shot (green, gray) and F-actin (red, gray). (B) Distribution of Shot between cap and intercap regions. Five embryos for each genotype. Whiskers indicate SD. Mann-Whitney test, **P < 0.01. (C) Fixed embryos of indicated genotype stained for Canoe (red, gray) and Par-1-GFP (green, gray). Two exemplary par-1 RNAi embryos are shown to indicate phenotypic variation. Position and range of axial projections as indicated. Lines in red indicate exemplary positions of line profiles. (D) Normalized line profiles from intercap region toward caps (interphase 13) for indicated genotypes. 60 line profiles in five embryos for each genotype. The solid line indicates average, band, and SD. Scale bars 10 µm.

Mutual antagonism between Par-1 and Patronin/Shot. (A) Fixed embryos of indicated genotypes and stage stained for Shot (green, gray) and F-actin (red, gray). (B) Distribution of Shot between cap and intercap regions. Five embryos for each genotype. Whiskers indicate SD. Mann-Whitney test, **P < 0.01. (C) Fixed embryos of indicated genotype stained for Canoe (red, gray) and Par-1-GFP (green, gray). Two exemplary par-1 RNAi embryos are shown to indicate phenotypic variation. Position and range of axial projections as indicated. Lines in red indicate exemplary positions of line profiles. (D) Normalized line profiles from intercap region toward caps (interphase 13) for indicated genotypes. 60 line profiles in five embryos for each genotype. The solid line indicates average, band, and SD. Scale bars 10 µm.

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