Par-1-GFP in intercaps depends on Kinesin-1. Living embryos with indicated genotype and stage expressing Par-1-GFP. (A) Images from movies. Rectangles in yellow mark the position of insets in higher magnification. Orthogonal view from reconstructions of axial image stacks. Dashed circles in white indicate selected nuclei. These are projection images from the time-lapse series shown in Fig. S4, A and B. (B) Intercap fluorescence of Par-1-GFP. 40 intercap regions in five embryos for each genotype. (C) Exemplary line profiles of Par-1-GFP in intercap regions. (D) Variance of line profiles. 52 lines in four embryos for each genotype. Whiskers indicate SD, Mann-Whitney test, ****P < 0.0001, scale bars 10 µm.