Mutual interactions between Par-1 and Patronin. (A) Fixed embryos with indicated genotypes and stage stained for Patronin-YFP (green, gray) and F-actin (red, gray). Arrows in yellow point to Patronin clusters. (B and C) Turnover of α-Tubulin assayed by FRAP in embryos of indicated genotypes. Photobleached areas are indicated by circles in yellow. (B) Exemplary series of images from movies. (C) Fluorescence within bleached areas normalized to last pre-bleaching value. Average (solid line) and SD (band) from six embryos for each genotype. (D and E) Microtubule dynamics assayed by tracking of growing plus-ends (EB1-GFP) in embryos of indicated genotypes. (D) Exemplary images from movies. Arrows in red point to growing end. (E) Velocities calculated from tracking growing ends. 22 measurements for each genotype. Whiskers indicate SD. Mann-Whitney test, statistically not significant, n.s. (F and H) Exemplary images from movies in widefield optics of embryos with indicated genotypes during interphase 14/cellularization. Arrows in yellow point to the tip of the ingressing furrow. Arrows in red point to nuclei. T = 0 when new nuclei appear during interphase 14/cellularization. (G) Living embryos with indicated genotypes expressing EB1-GFP. Exemplary images from movies, position, and range of axial projections as indicated. Arrows in yellow point to ectopic EB1-GFP signal in the intercap region. (I and J) Fixed embryos of indicated genotypes in interphase 14/cellularization stained for (I) Bazooka (green, gray) and Slam (red, gray), sagittal sections or (J) Canoe (red, gray) and Par-1-GFP (green, gray). Position and range of axial projections as indicated. Images from two exemplary patronin RNAi embryos to show phenotypic variation. Scale bars 10 µm.