Cortical accumulation of Par-1 depends on Kinesin1. (A–D) Living embryos with indicated genotypes expressing (A–C) Par-1-GFP or (D) EB1-GFP and Moesin-RFP. Arrays of images from movies with axial position, range, and time/stage as indicated. (C) Images from axial stacks to demonstrate the phenotypic variation in Kinesin-1 RNAi embryos. (D) Images from axial stacks in early interphase 13. Scale bars 10 µm.