Cortical Patronin, Shot, and EB1 depend on Kinesin-1. (A) Centrosomal and non-centrosomal microtubules at the cortex during syncytial interphase. (B, C, F, and G) Living embryos of indicated genotypes expressing the F-actin label Moesin-RFP and Patronin-YFP (B and C) or EB1-GFP (F and G). (B) Yellow arrows point to Patronin clusters. (C) Patronin-YFP clusters within the cap region. 21 caps in three embryos for each genotype. (D and E) Fixed embryos stained for Shot (green, gray) and F-actin (red, gray). (E) Ratio of number of Shot clusters in cap versus intercap region. Four (wild-type) or five (Kinesin-1 RNAi) embryos were scored. (F) Arrows in yellow point to EB1 signal within the intercap domain. Axial position and ranges of projections as indicated. These are projection images from the time-lapse series shown in Fig. S4 D. (G) Fluorescence within intercap region normalized to total fluorescence. 24 regions in four embryos for each genotype. Whiskers indicate SD, Mann-Whitney test, statistically not significant, n. s. *P < 0.05, ****P < 0.0001, scale bars 10 µm.