Rho signaling and Par-1 at the intercap region. (A–C) Living embryos expressing Pebble-GFP (green, gray) and Sqh-mKate (red, gray) during interphase 13. Position and range of projections as indicated. (A) Axial image stack. (B) Axial projections as indicated. The line in red indicates an exemplary position of line profiles across the intercap region. (C) Averaged line profiles positioned by maximum of Pebble-GFP profiles. (D) Western blotting for Pebble with extracts from embryos with indicated genotypes. α-Tubulin, loading control. (E and F) Living embryos with indicated genotypes expressing Rho-GFP sensor. Position of focus as indicated. (F) Total fluorescence of Rho sensor scored in six wild-type and seven pebble RNAi embryos. (G–H) Living embryos of indicated genotypes in indicated stage expressing Par-1-GFP. (G) Surface view and reconstructed axial section with selected nuclei marked by dashed lines. To compare the effect of pebble and Kinesin-1 on Par-1-GFP accumulation, wild-type images were used in both Fig. 7 A and here. (H) Par-1-GFP fluorescence scored in six wild-type, six Kinesin-1 RNAi, and seven pebble RNAi embryos. Whiskers indicate SD, Mann-Whitney test, **P < 0.01, statistically not significant, n.s., scale bars 10 µm. Source data are available for this figure: SourceData FS3.