Rho signaling via Pebble depends on Kinesin-1. (A–E) Living embryos with indicated genotypes expressing Pebble-GFP (A–C) or Rho sensor (D and E). (A) Images from movies in interphase 13. The rectangle in red indicates an exemplary position of a line profile across the intercap region. (B) 30 line profiles in three embryos for each genotype. Average (solid line) and SEM (band). Normalized and positioned to peak in wild type. (C) Pebble-GFP fluorescence within the intercap region. 52 measurements in four embryos for each genotype. (D) Orthogonal view from reconstructions of axial image stacks with indicated genotype at indicated time. Dashed lines indicated selected nuclei. (E) Fluorescence of Rho sensor within intercap region. 40 measurements in four embryos for each genotype. T = 0 when Rho sensor signal appears between daughter nuclei. Whiskers indicate SD, Mann-Whitney test, ****P < 0.0001, scale bars 10 µm.