Figure 4.
Cpa accumulation at intercap regions depends on Kinesin-1. (A and B) Images from fixed embryos stained for Cpa (green, gray) and F-actin (red, gray). Wild-type embryos were labeled with coexpression of Histon2Av-GFP. Stage and genotype as indicated. Surface view and sagittal sections. Line in yellow indicates positioning of line profiles. (C) Normalized line profiles from intercap region toward caps (interphase 13) for indicated genotypes. The solid line indicates average, band, and standard error of the mean (SEM). 20 line profiles in four embryos for each genotype. (D) Relative total fluorescence at the cortex for F-actin and Cpa. F-actin: 20 (wild type) and 24 (Kinesin-1 RNAi) regions in four embryos. Cpa, 16 measurements in four embryos of each genotype. Whiskers indicate SD, Mann-Whitney test, n.s., statistically not significant, ****P < 0.0001, scale bar 10 µm.

Cpa accumulation at intercap regions depends on Kinesin-1. (A and B) Images from fixed embryos stained for Cpa (green, gray) and F-actin (red, gray). Wild-type embryos were labeled with coexpression of Histon2Av-GFP. Stage and genotype as indicated. Surface view and sagittal sections. Line in yellow indicates positioning of line profiles. (C) Normalized line profiles from intercap region toward caps (interphase 13) for indicated genotypes. The solid line indicates average, band, and standard error of the mean (SEM). 20 line profiles in four embryos for each genotype. (D) Relative total fluorescence at the cortex for F-actin and Cpa. F-actin: 20 (wild type) and 24 (Kinesin-1 RNAi) regions in four embryos. Cpa, 16 measurements in four embryos of each genotype. Whiskers indicate SD, Mann-Whitney test, n.s., statistically not significant, ****P < 0.0001, scale bar 10 µm.

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