Myosin II and Dia accumulation at the intercap region partially depend on Kinesin-1. (A) Scheme of Rho signaling with Myosin II, Dia, and Cpa as direct and indirect targets. (B and D) Orthogonal view from reconstructions of axial image stacks with indicated genotype and markers: Sqh-GFP/Myosin II (B), Dia-GFP (D). Selected nuclei indicated by dashed lines. (C and E) Fluorescence of Sqh-GFP (C) and Dia-GFP (E) within the intercap domain; 33 intercap regions in three embryos for each genotype (C), 30 intercap regions in three embryos for each genotype (E). T = 0 when Sqh-GFP and Dia-GFP signal appears between daughter nuclei. Whiskers indicate SD, Mann-Whitney test, ****P < 0.0001, scale bars 10 µm.