Figure S2.
Cortical Myosin II and Dia depend on Kinesin-1. (A and B) Living embryos with indicated genotypes expressing the Rho1 sensor in interphase 13. (A) Dynamics of the width of the region with Rho sensor. 30 regions in three embryos of each genotype. (B) Fluorescence of the Rho sensor. 52 regions in four embryos of each genotype. (C–J and L–O) Living embryos of indicated genotype expressing Sqh-GFP (Myosin II regulatory light chain), Zipper-GFP (Myosin II heavy chain), or Dia-GFP during interphase 13 and interphase 14 as indicated. Sagittal views are from reconstructions of axial stacks. Position and range of axial projections as indicated. (D) Total fluorescence of Sqh-GFP. (E) Sagittal reconstructions. Selected nuclei are indicated by dashed lines. (F) Sqh-GFP fluorescence at the tip of the furrow. 33 regions in three embryos for each genotype. (G–J) Zipper-GFP during interphase 13. (H) Total fluorescence. Six (wild-type) and four (Kinesin-1 RNAi) embryos were scored. (I) Sagittal reconstructions from movies. Selected nuclei indicated by dashed lines. (J) Fluorescence at intercap regions. 60 regions in three embryos (wild type) and 72 regions in four embryos (Kinesin-1 RNAi) were scored at T = 2 min of interphase 13. (K) Fixed embryos of indicated genotype stained for Zipper. Surface and sagittal sections. (L–O) Dia-GFP fluorescence in interphase 13 and 14. (M) Total fluorescence. Interphase 13: four (wild type), three (Kinesin-1 RNAi). Interphase 14: three embryos for each genotype. (N) Sagittal reconstructions. Dashed lines indicated selected nuclei. (O) Dia-GFP fluorescence at the tip of furrows. 30 furrow tips in three embryos for each genotype at T = 0, interphase 14. (P–R) Western blots with extracts from embryos of indicated genotypes for (P and Q) Dia or (R) Cpa with α-Tubulin as loading control. T = 0 when Rho sensor, Sqh-GFP, Zipper-GFP, and Dia-GFP signal appears between daughter nuclei. Whiskers indicate SD. For M, unpaired t test, *P < 0.05, **P < 0.01, for others, Mann-Whitney test, *P < 0.05, **P < 0.01, ****P < 0.0001, scale bars 10 µm. Source data are available for this figure: SourceData FS2.

Cortical Myosin II and Dia depend on Kinesin-1. (A and B) Living embryos with indicated genotypes expressing the Rho1 sensor in interphase 13. (A) Dynamics of the width of the region with Rho sensor. 30 regions in three embryos of each genotype. (B) Fluorescence of the Rho sensor. 52 regions in four embryos of each genotype. (C–J and L–O) Living embryos of indicated genotype expressing Sqh-GFP (Myosin II regulatory light chain), Zipper-GFP (Myosin II heavy chain), or Dia-GFP during interphase 13 and interphase 14 as indicated. Sagittal views are from reconstructions of axial stacks. Position and range of axial projections as indicated. (D) Total fluorescence of Sqh-GFP. (E) Sagittal reconstructions. Selected nuclei are indicated by dashed lines. (F) Sqh-GFP fluorescence at the tip of the furrow. 33 regions in three embryos for each genotype. (G–J) Zipper-GFP during interphase 13. (H) Total fluorescence. Six (wild-type) and four (Kinesin-1 RNAi) embryos were scored. (I) Sagittal reconstructions from movies. Selected nuclei indicated by dashed lines. (J) Fluorescence at intercap regions. 60 regions in three embryos (wild type) and 72 regions in four embryos (Kinesin-1 RNAi) were scored at T = 2 min of interphase 13. (K) Fixed embryos of indicated genotype stained for Zipper. Surface and sagittal sections. (L–O) Dia-GFP fluorescence in interphase 13 and 14. (M) Total fluorescence. Interphase 13: four (wild type), three (Kinesin-1 RNAi). Interphase 14: three embryos for each genotype. (N) Sagittal reconstructions. Dashed lines indicated selected nuclei. (O) Dia-GFP fluorescence at the tip of furrows. 30 furrow tips in three embryos for each genotype at T = 0, interphase 14. (P–R) Western blots with extracts from embryos of indicated genotypes for (P and Q) Dia or (R) Cpa with α-Tubulin as loading control. T = 0 when Rho sensor, Sqh-GFP, Zipper-GFP, and Dia-GFP signal appears between daughter nuclei. Whiskers indicate SD. For M, unpaired t test, *P < 0.05, **P < 0.01, for others, Mann-Whitney test, *P < 0.05, **P < 0.01, ****P < 0.0001, scale bars 10 µm. Source data are available for this figure: SourceData FS2.

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