Rho sensor dynamics in interphase 13 depend on Kinesin-1. (A) Images from movies of Utrophin-GFP during interphase 13. T = 0 when new actin caps formed during interphase 13 . Genotypes as indicated. (B) Images from movies for Rho sensor during mitosis 12 and interphase 13. Arrows in yellow point to intercap region. (C) Total cortical fluorescence of the Rho sensor intensity. Six embryos for each genotype. (D) Orthogonal view from reconstructions of axial image stacks. Selected nuclei marked by dashed lines. (E) Fluorescence of Rho sensor at the intercap region. 52 intercap regions in four embryos for each genotype. T = 0 when Rho sensor signal appears between daughter nuclei. Whiskers indicate SD, Mann-Whitney test, **P < 0.01, ****P < 0.0001, scale bars 10 µm.