Cellularization during interphase 14 depends on Kinesin-1. (A) Images from movies of embryos with indicated genotypes in widefield optics. Kin1 germline indicates embryos from Khc27 germline clones. Arrows in yellow point to the basal tip of the ingressing plasma membrane (cellularization furrows). Kin1-GFP are embryos expressing Kinesin-1-GFP in addition to endogenous Kinesin-1. T = 0 when new nuclei appear during interphase 14/cellularization. (B and C) Living embryos with indicated genotypes expressing Kinesin-1-mKate during interphase 13. (B) Position and range of axial projections as indicated. (C) Total fluorescence of Kinesin-1-mKate. Eight embryos for each genotype. Mann-Whitney test, ***P < 0.001. (D and E) Extracts from embryos with indicated genotypes analyzed by western blotting for content of Kinesin-1 protein and α-Tubulin as loading control. (E) Signals in western blots normalized to α-Tubulin. Three (wild-type) and four (Kinesin1 RNAi) biological replicates. Unpaired t-test, **P < 0.01. (F and G) Fixed embryos of indicated genotypes stained for (F) Dlg (green, gray) and Slam (red) (sagittal section) or (G) Canoe (red, gray), Slam (green, gray), and DNA (blue). Whiskers indicate SD, scale bars 10 µm. Source data are available for this figure: SourceData FS1.
