Cell motility parameters based on cell cycle phase and track location. (A) Histogram of cell tracks of different duration in all time-lapse imaging videos (n^S/G2/M = 3,191, n^G1/G0 = 6,584 from seven experiments). (B–D) Comparison of the mean velocity (B), net velocity (C), and straightness (D) of tracks of S/G2/M and G1/G0 phase cells (n^S/G2/M = 3,191, n^G1/G0 = 6,584, seven experiments). (E and F) Comparison of the net velocity (E) and straightness (F) of tracks within tip and duct regions (see schematics in Fig. 5 for how regions were defined) of nine elongating branches (n^tip = 600, n^duct = 1,814), four experiments. (G and H) Velocity of tracks within bifurcating tips that were closer to the future cleft (G) or closer to future tips (H) at different time points relative to the appearance of the cleft at T0 (n^tip = 2,058, n^cleft = 1,499 from 10 videos, seven experiments). (I and J) Net velocity (I) and straightness (J) of cell tracks within bifurcating tips, divided in those closer to the future cleft and those closer to the future daughter tips, compared those within the duct (boundary between parent tip and duct defined based on curvature of the neck), based on whether they started before bifurcation (n^tip = 272, n^cleft = 77, n^duct = 154), during tip widening (n^tip = 688, n^cleft = 279, n^duct = 1,152) or after cleft formation (n^tip = 1,098, n^cleft = 1,143, n^duct = 1,645). Correlation between variables in G and H was assessed with the Pearson coefficient (R) and P value for the linear correlation given. Data shown in B–F, I, and J represents the median (line) with 25th and 75th percentiles (hinges) plus 1.5× interquartile ranges (whiskers). Statistical significance was assessed with two-tailed t test (E and F) and Wilcoxon’s rank sum test (B–D, I, and J); *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.