TEAD-associated signaling is activated under 5SA-YAP and 5SA-CCDEL-YAP but not 5SA-S94A-YAP overexpression. mRNA expression of the TEAD targets Cyr61 and CTGF in high-density cells subjected to constitutive overexpression of an empty vector (control), 5SA-YAP, 5SA-CCDDEL-YAP, and 5SA-S94A-YAP, measured by RT-qPCR. The values refer to the fold change compared to the control condition. GAPDH was used as the housekeeping gene. The graphs represent the mean of n = 5–6 biological repeats (black dots), and the error bars represent the SD. Ordinary one-way ANOVA test; **** indicates P value <0.0001; ** indicates P value <0.01, * indicates P value <0.1; ns indicates P value >0.5.