Figure S5.
Expression of PIKfyve-GFP in Dictyostelium. (A) Localization of PIKfyve-GFP in WT cells. Boxes indicate regions enlarged in the panels on the right; arrowhead indicates perinuclear cluster of vesicles. (B) Rescue of the macropinosome concentration defect by PIKfyve-GFP expression in ΔPIKfyve cells. The cell lines indicted were incubated overnight in Texas-Red dextran overnight prior to confocal microscopy. Scale bar = 2 μm.

Expression of PIKfyve-GFP in Dictyostelium. (A) Localization of PIKfyve-GFP in WT cells. Boxes indicate regions enlarged in the panels on the right; arrowhead indicates perinuclear cluster of vesicles. (B) Rescue of the macropinosome concentration defect by PIKfyve-GFP expression in ΔPIKfyve cells. The cell lines indicted were incubated overnight in Texas-Red dextran overnight prior to confocal microscopy. Scale bar = 2 μm.

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