Recruitment of SnxA relative to PI(3)P and PIKfyve. (A) Representative timelapse of cells co-expressing 2xFYVE-RFP (magenta) and SnxA-GFP (green) during phagocytosis of an unlabeled yeast. Engulfed yeast is indicated with an asterisk; scale bar = 2 μm. See Video 4 for full-time series. (B) Quantification of the fluorescence intensities of both channels around the yeast in this video. (C and D) Recruitment of GFP-PIKfyve to a newly formed macropinosome and phagosome, respectively. The full timelapse is shown in Videos 5 and 6. (E) Timelapse of cells co-expressing 2xPX-RFP (magenta) and PIKfyve-GFP (green) during phagocytosis of Alexa405-yeast (blue). (F) Quantification of the fluorescence intensities around the phagosome in E. Data shown is the mean intensity ± the SD around the phagosomal membrane normalized between minimum and maximum intensities observed.