Loss of SnxA has little effect on phagosome or macropinosome maturation. (A) Images of WT and ΔsnxA-1 colonies growing on a lawn of K. aerogenes. Average colony diameter over time is shown below. Scale bar = 10 mm. (B) Growth of mutant cells in different bacteria. Numbers indicate the number of Dictyostelium cells seeded on each well. (C) Summary of growth on a range of bacteria screened as in B. (D) Quantification of endocytosis calculated by FITC dextran uptake. (E) Phagocytosis of 1 μm fluorescent beads, measured by flow cytometry and normalized to WT cells at 60 min. (F and G) Phagosomal proteolysis (F) and pH (G) over time, measured by fluorescence changes after engulfment of either DQ-BSA or FITC/Alexa594 reporter beads, respectively. (H) Representative images of macropinosomes shrinking after a 2-min (mins) pulse of TRITC-dextran; scale bar = 2 μm. (I) Size is quantified. All data shown are the mean ± SD of at least three independent experiments. ΔsnxA-1, 2, and 3 indicate independent knockout clones.