Figure 2. PI(3,5)P 2 binding... | Rockefeller University Press

Figure 2.

PI(3,5)P2binding specificity. (A–D) Representative images and quantification of binding of recombinant GFP-SnxA probes to membrane-coated beads containing the indicated PIPs. (E) Binding of each SnxA probe titrated against beads harboring PI(3,5)P2. (F) Intensities measured under identical imaging conditions and apparent Kds. All graphs show the mean ± SD from three independent experiments; scale bars indicate 5 μm.

PI(3,5)P ₂ binding specificity. (A–D) Representative images and quantification of binding of recombinant GFP-SnxA probes to membrane-coated beads containing the indicated PIPs. (E) Binding of each SnxA probe titrated against beads harboring PI(3,5)P₂. (F) Intensities measured under identical imaging conditions and apparent K_ds. All graphs show the mean ± SD from three independent experiments; scale bars indicate 5 μm.