Figure S1.
SnxA does not bind PI(5)P. (A) PIP array performed using lysates from WT cells expressing 2xPX-GFP and probed with anti-GFP antibody. (B) Coomassie-stained SDS-PAGE gels of recombinant GFP fusion SnxA probes used for lipid binding assays. (C) Representative images of SnxA probe titrated against PI(5)P-containing membrane-coated beads. (D) This is quantified and plotted as the mean ± SD from three independent experiments. (E) Mass photometry analysis of recombinant SnxA fusions. Note that exact molecular weights of native proteins are inaccurate below ∼100 kD using this technique. Source data are available for this figure: SourceData FS1.

SnxA does not bind PI(5)P. (A) PIP array performed using lysates from WT cells expressing 2xPX-GFP and probed with anti-GFP antibody. (B) Coomassie-stained SDS-PAGE gels of recombinant GFP fusion SnxA probes used for lipid binding assays. (C) Representative images of SnxA probe titrated against PI(5)P-containing membrane-coated beads. (D) This is quantified and plotted as the mean ± SD from three independent experiments. (E) Mass photometry analysis of recombinant SnxA fusions. Note that exact molecular weights of native proteins are inaccurate below ∼100 kD using this technique. Source data are available for this figure: SourceData FS1.

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