Figure 2.
FN inhibits RA formation in a local manner. (A) U2Os-AP2-GFP cells plated on FN, VTN, Col I, Col IV, LN111 (all 10 µg/ml) -coated or non-coated dishes, stained for p-Pax and integrin αvβ. Representative TIRF images. (B) Analysis of RA coverage from samples in A. N = 21 from two independent experiments. F(5, 120) = 48.05, P < 0.0001. (C) U2Os-AP2-GFP cells were plated on FN/glass patterned imaging dishes overnight, stained for integrin αvβ5 and p-Pax, and imaged using TIRF microscopy; representative TIRF images. (D) Quantification of integrin αvβ5 fluorescent intensity on FN- and glass-side of the pattern; n = 10 from one representative experiment. Similar results were observed in five similar experiments. Two-tailed Student’s t test, P < 0.01. (E) A schematic illustration of the results shown in this figure. Data are the mean ± SD, ns. non-significant P value; *** P value < 0.001. Scale bars, 10 µm; insets, 5 µm.

FN inhibits RA formation in a local manner. (A) U2Os-AP2-GFP cells plated on FN, VTN, Col I, Col IV, LN111 (all 10 µg/ml) -coated or non-coated dishes, stained for p-Pax and integrin αvβ. Representative TIRF images. (B) Analysis of RA coverage from samples in A. N = 21 from two independent experiments. F(5, 120) = 48.05, P < 0.0001. (C) U2Os-AP2-GFP cells were plated on FN/glass patterned imaging dishes overnight, stained for integrin αvβ5 and p-Pax, and imaged using TIRF microscopy; representative TIRF images. (D) Quantification of integrin αvβ5 fluorescent intensity on FN- and glass-side of the pattern; n = 10 from one representative experiment. Similar results were observed in five similar experiments. Two-tailed Student’s t test, P < 0.01. (E) A schematic illustration of the results shown in this figure. Data are the mean ± SD, ns. non-significant P value; *** P value < 0.001. Scale bars, 10 µm; insets, 5 µm.

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