Figure S3.
Wounding triggers the enrichment of the Golgi membrane at the wound site, related toFig. 3. (A) Representative single-plane confocal images showing the localization of the Golgi apparatus (stained with Golgi-tracker-red) before and after wounding in N2 worms. “W” indicates the wound site. Scale bar: 10 µm. (B) Representative single-plane confocal images showing the colocalization of TGN-38::tagBFP, GFP::MANS-2, and mKate2::R12B2.2 before and after wounding. Pcol-19-TGN-38::tagBFP(zjuSi280); Pcol-19-GFP::MANS-2(zjuSi302); Pcol-19-mKate2::R12B2.2(zjuEx2075) transgenic animals were used for wounding and imaging. Magenta arrows indicate the colocalized puncta. Scale bar: 10 µm. (C) Representative HiS-SiM single-plane images showing the colocalization of PH::GFP and TGN-38::tagBFP before and after wounding. Pcol-19-PH::GFP(zjuSi175); Pcol-19-TGN-38::tagBFP(zjuSi280) transgenic animals were used for wounding and imaging. “W” indicates the wound site. Scale bar: 10 µm. (D) Representative confocal images of mKate2::P4M with the treatment of rab-1 RNAi, rab-6.2 RNAi, and BFA (150 µg/ml), as well as in rab-6.2(ok2254) mutant animals before and after wounding. Pcol-19-mKate2::P4M(zjuSi333) transgenic animals were used for drug treatment, Pcol-19-mKate2::P4M(zjuSi333); Pcol-19-RDE-1(juIs346); rde-1(ne219) for RNAi treatments. Scale bar: 10 µm. (E) Quantitation analysis of the intensity ratio (ΔFw/Fuw) for the different treatments shown in (E). The error bars represent the mean value ± SD (n = 156, 19, 22, 138, 134 from the left to the right), Mann–Whitney test, ***P < 0.001. **P < 0.01. (F) Representative confocal images showing TGN-38::tagBFP and mKate2::P4M with the treatment of GSK-A1 (100 μM) before and after wounding. Pcol-19-TGN-38::tagBFP(zjuSi280); Pcol-19-mKate2::P4M(zjuSi333) transgenic animals were used for wounding and imaging. Scale bar: 10 µm. (G) Representative single-plane confocal images showing the colocalization of mKate2::ZC8.6 with TGN-38::tagBFP or GFP::MANS-2. Pcol-19-TGN-38::tagBFP(zjuSi280); Pcol-19-mKate2::ZC8.6(zjuEx2330), Pcol-19-GFP::MANS-2(zjuSi302); Pcol-19-mKate2::ZC8.6(zjuEx2320) transgenic animals were used for wounding and imaging. Scale bar: 10 µm.

Wounding triggers the enrichment of the Golgi membrane at the wound site, related to Fig. 3 . (A) Representative single-plane confocal images showing the localization of the Golgi apparatus (stained with Golgi-tracker-red) before and after wounding in N2 worms. “W” indicates the wound site. Scale bar: 10 µm. (B) Representative single-plane confocal images showing the colocalization of TGN-38::tagBFP, GFP::MANS-2, and mKate2::R12B2.2 before and after wounding. Pcol-19-TGN-38::tagBFP(zjuSi280); Pcol-19-GFP::MANS-2(zjuSi302); Pcol-19-mKate2::R12B2.2(zjuEx2075) transgenic animals were used for wounding and imaging. Magenta arrows indicate the colocalized puncta. Scale bar: 10 µm. (C) Representative HiS-SiM single-plane images showing the colocalization of PH::GFP and TGN-38::tagBFP before and after wounding. Pcol-19-PH::GFP(zjuSi175); Pcol-19-TGN-38::tagBFP(zjuSi280) transgenic animals were used for wounding and imaging. “W” indicates the wound site. Scale bar: 10 µm. (D) Representative confocal images of mKate2::P4M with the treatment of rab-1 RNAi, rab-6.2 RNAi, and BFA (150 µg/ml), as well as in rab-6.2(ok2254) mutant animals before and after wounding. Pcol-19-mKate2::P4M(zjuSi333) transgenic animals were used for drug treatment, Pcol-19-mKate2::P4M(zjuSi333); Pcol-19-RDE-1(juIs346); rde-1(ne219) for RNAi treatments. Scale bar: 10 µm. (E) Quantitation analysis of the intensity ratio (ΔFw/Fuw) for the different treatments shown in (E). The error bars represent the mean value ± SD (n = 156, 19, 22, 138, 134 from the left to the right), Mann–Whitney test, ***P < 0.001. **P < 0.01. (F) Representative confocal images showing TGN-38::tagBFP and mKate2::P4M with the treatment of GSK-A1 (100 μM) before and after wounding. Pcol-19-TGN-38::tagBFP(zjuSi280); Pcol-19-mKate2::P4M(zjuSi333) transgenic animals were used for wounding and imaging. Scale bar: 10 µm. (G) Representative single-plane confocal images showing the colocalization of mKate2::ZC8.6 with TGN-38::tagBFP or GFP::MANS-2. Pcol-19-TGN-38::tagBFP(zjuSi280); Pcol-19-mKate2::ZC8.6(zjuEx2330), Pcol-19-GFP::MANS-2(zjuSi302); Pcol-19-mKate2::ZC8.6(zjuEx2320) transgenic animals were used for wounding and imaging. Scale bar: 10 µm.

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