Figure S1. Epidermal membrane wounding... | Rockefeller University Press

Figure S1.

Epidermal membrane wounding triggers PtdIns4P accumulation and PtdIns(4,5)P2generation at the wound site, related toFig. 1. (A) Representative single-plane confocal images showing the colocalization of myr::mKate2 and PH::GFP before and after wounding. Pcol-19-myr::mKate2(zjuSi46); Pcol-19-PH::GFP(zjuSi175) transgenic animals were used for wounding and imaging. “W” indicates the wound site. White dotted boxes indicate the zoom-in region. Blue arrows pointed to the colocalized puncta, green arrows pointed to PH::GFP puncta, and magenta arrows pointed to myr::mKate2 puncta. Scale bar: 10 µm. (B) HiS-SiM time-lapse images of PH::GFP in wounded Pcol-19-PH::GFP(zjuSi175) animals. Scale bar: 10 µm. (C) Representative single-plane confocal images showing the colocalization of mKate2::P4M and PH::GFP before and after wounding. Pcol-19-PH::GFP(zjuSi175); Pcol-19-mKate2::P4M(zjuSi333) transgenic animals were used for wounding and imaging. White dotted boxes indicate the zoom-in region. Magenta arrows indicate the colocalized puncta. Scale bar: 10 µm. (D) Line graph showing the intensity change (ΔFw/Fuw) of unwounded and wounded worms after FRAP. The mean ± SD at each time point is indicated (n = 27, 18, 27, 18 from the top to the bottom).

Epidermal membrane wounding triggers PtdIns4P accumulation and PtdIns(4,5)P ₂ generation at the wound site, related to Fig. 1 . (A) Representative single-plane confocal images showing the colocalization of myr::mKate2 and PH::GFP before and after wounding. Pcol-19-myr::mKate2(zjuSi46); Pcol-19-PH::GFP(zjuSi175) transgenic animals were used for wounding and imaging. “W” indicates the wound site. White dotted boxes indicate the zoom-in region. Blue arrows pointed to the colocalized puncta, green arrows pointed to PH::GFP puncta, and magenta arrows pointed to myr::mKate2 puncta. Scale bar: 10 µm. (B) HiS-SiM time-lapse images of PH::GFP in wounded Pcol-19-PH::GFP(zjuSi175) animals. Scale bar: 10 µm. (C) Representative single-plane confocal images showing the colocalization of mKate2::P4M and PH::GFP before and after wounding. Pcol-19-PH::GFP(zjuSi175); Pcol-19-mKate2::P4M(zjuSi333) transgenic animals were used for wounding and imaging. White dotted boxes indicate the zoom-in region. Magenta arrows indicate the colocalized puncta. Scale bar: 10 µm. (D) Line graph showing the intensity change (ΔF_w/F_uw) of unwounded and wounded worms after FRAP. The mean ± SD at each time point is indicated (n = 27, 18, 27, 18 from the top to the bottom).